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KMID : 0043319900130020174
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Archives of Pharmacal Research
1990 Volume.13 No. 2 p.174 ~ p.179
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Determination of Total Glycyrrhetic Acid in Glycyrrhizae Radix by Second Derivative UV Spectrometry
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Song SB
Choi JK/Yoo GS
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Abstract
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Second derivative (D2) spectrometry using ion-pair extraction technique was developed for the determination of total glycyrrhetic acid (GA) in Glycyrrhizae Radix. Glycyrrhizin (G) obtained from Glycyrrhizae Radix was hydrolyzed into GA in 2 N-HCl and methanol (1:1) and extracted from aqueous phase in the form of an ion-pair complex with tetrapentylammonium bromide (TPA) as a counter ion. Maximum D2 amplitude (Z value) was obrained when 1000-fold or greater molar ratio of TPA was used at pH 11. Reaction time, temerature and ionic strength did not affect ion-pair formation. Dichloromethane was an effective extraction solvent of the ion-pair complex. The linearity of standard curve of ion-pair GA was obtained in the range of 4-120mcg/ml as GA. Assayed contents of GA in dry powder by D2 UV spectrometry and HPLC method were 5.31 +/- 0.04% and 5.20 +/- 0.008 %, respectively.
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